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Expression and identification of immunity determinants on linear DNA killer plasmids pGKL1 and pGKL2 in Kluyveromyces lactis.

机译:乳酸克鲁维酵母中线性DNA杀伤质粒pGKL1和pGKL2的免疫决定簇的表达和鉴定。

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摘要

The linear dsDNA plasmids, pGKL1 (8.9 kb) and pGKL2 (13.4 kb) discovered in Kluyveromyces lactis, confer killer and immunity characteristics upon various yeast strains. We have devised an immunity assay and have been able to show the expression of an immunity phenotype in the K. lactis transformants harbouring conventional circular plasmids which contain DNA fragments of pGKL1. Using this expression system, the immunity determinant on pGKL1 was identified as ORF5. In addition, the presence of pGKL2 was proved to be essential for the expression of the immunity phenotype. This is the first demonstration of this new pGKL2 function, as distinct from its known functions for the replication and maintenance of pGKL1 in yeast cells.
机译:在乳酸克鲁维酵母中发现的线性dsDNA质粒pGKL1(8.9 kb)和pGKL2(13.4 kb),赋予各种酵母菌株杀伤力和免疫力。我们已经设计了一种免疫测定法,并且已经能够显示出具有包含pGKL1 DNA片段的常规环状质粒的乳酸克鲁维酵母转化体中免疫表型的表达。使用该表达系统,将对pGKL1的免疫决定簇鉴定为ORF5。另外,pGKL2的存在被证明对于表达免疫表型是必不可少的。这是这种新的pGKL2功能的首次展示,与已知的在酵母细胞中复制和维持pGKL1的功能不同。

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